What does Safranin coloring do
Safranin T (arab. zafaran, Yellowness) is a derivative of phenazine. It is a member of the Safranine series, with colors between red and purple.
Extraction and presentation
The dye is made synthetically from a mixture of o-toluidine, aniline with hydrochloric acid and sodium nitrite.
Safranin T is used for staining purposes in microscopy. Also as a dye for leather. Also used as a pH and redox indicator in chemical analysis. The oxidized form is red, the reduced form is colorless. It can also be used for Gram staining of bacteria. In addition, it is used in a double coloration with astra blue, whereby it colors lignified cell walls red, while unwooded cell walls are colored blue with astrab blue.
- ↑ Since December 1, 2012, only GHS hazardous substance labeling has been permitted for substances. Until June 1, 2015, the R-phrases of this substance may still be used to classify preparations, after which the EU hazardous substance labeling is of purely historical interest.
- ↑ 3,03,13,23,3Norbert Welsch in: Römpp Online - Version 3.5, 2009, Georg Thieme Verlag, Stuttgart.
- ↑ F. W. Küster, A. Thiel, A. Ruland: Calculation tables for chemical analysis, P. 79, 105th edition, de Gruyter Verlag, 2002, ISBN 3-11-017566-5.
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