What does Safranin coloring do

Safranin T

Template: Infobox chemical / molecular formula search available

Safranin T (arab. zafaran, Yellowness) is a derivative of phenazine. It is a member of the Safranine series, with colors between red and purple.

Extraction and presentation

The dye is made synthetically from a mixture of o-toluidine, aniline with hydrochloric acid and sodium nitrite.[3]


Safranin T is used for staining purposes in microscopy.[3] Also as a dye for leather.[3] Also used as a pH and redox indicator in chemical analysis.[3] The oxidized form is red, the reduced form is colorless.[4] It can also be used for Gram staining of bacteria. In addition, it is used in a double coloration with astra blue, whereby it colors lignified cell walls red, while unwooded cell walls are colored blue with astrab blue.

Individual evidence

  1. 1,01,11,21,31,4Entry to 3,7-diamino-2,8-dimethyl-5-phenylphenazinium chloride in the GESTIS substance database of the IFA, accessed on January 19, 2008 (JavaScript required)
  2. ↑ Since December 1, 2012, only GHS hazardous substance labeling has been permitted for substances. Until June 1, 2015, the R-phrases of this substance may still be used to classify preparations, after which the EU hazardous substance labeling is of purely historical interest.
  3. 3,03,13,23,3Norbert Welsch in: Römpp Online - Version 3.5, 2009, Georg Thieme Verlag, Stuttgart.
  4. ↑ F. W. Küster, A. Thiel, A. Ruland: Calculation tables for chemical analysis, P. 79, 105th edition, de Gruyter Verlag, 2002, ISBN 3-11-017566-5.

See also

Web links